Bat ASC2 suppresses inflammasomes and ameliorates inflammatory diseases.

Bats are special in their ability to live long and host many emerging viruses. Our previous studies showed that bats have altered inflammasomes, which are central players in aging and infection. However, the role of inflammasome signaling in combating inflammatory diseases remains poorly understood. Here, we report bat ASC2 as a potent negative regulator of inflammasomes. Bat ASC2 is highly expressed at both the mRNA and protein levels and is highly potent in inhibiting human and mouse inflammasomes. Transgenic expression of bat ASC2 in mice reduced the severity of peritonitis induced by gout crystals and ASC particles. Bat ASC2 also dampened inflammation induced by multiple viruses and reduced mortality of influenza A virus infection. Importantly, it also suppressed SARS-CoV-2-immune-complex-induced inflammasome activation. Four key residues were identified for the gain of function of bat ASC2. Our results demonstrate that bat ASC2 is an important negative regulator of inflammasomes with therapeutic potential in inflammatory diseases.

Selection and evaluation of environmentally friendly pesticides, biological preparations and resistant variety samples against harmful objects of gourds in the conditions of the south-east of Kazakhstan

In the Republic of Kazakhstan melons and gourds are produced mainly in the traditional way, that is, by using chemical fertilizers and pesticides. The production of environmentally friendly melons is at the initial stage of its development. There is no organic melon growing at all. Considering the huge export potential of melons among other crops cultivated in the country (the total supply of melons and melons on the domestic market is 472%), the great economic and social significance of the transition of the melon industry to organic production is growing. In the future, Kazakhstan may be in the center of attention of the world community as a manufacturer and supplier of environmentally friendly (organic) melons, and environmentally friendly, natural melons may well become a brand of our sovereign state. To do this, it is necessary to make fundamental changes in the melon industry of the country. In general, the transition of agricultural production to an organic direction will contribute to the consumption of the safest and most useful products by the local population. Here, one should also take into account the decrease in the immunity of the human body due to various diseases, including those associated with the coronavirus pandemic. It should also be taken into account that only a limited number of pesticides used against harmful objects in agriculture act as intended, and most of the toxic substances spread into the environment. The use of disinfectants without scientific support has a negative impact on flora and fauna, being deposited in the soil and irrigation system of fields for a long time, thereby causing enormous damage to the environment. Given the above facts, our scientific research was aimed at the selection of pesticides and biological preparations with high biological and economic efficiency, low rates and frequency of treatments, as well as varieties with high resistance to fungal diseases of melons (watermelon, melon). Research work was carried out on the experimental plots of the Regional Branch "Kainar" of the LLP "Kazakh Research Institute of Horticulture" in the period 2020-2021. The studies used classical methods generally accepted in melon growing. The results obtained are new and relevant, and in the future, will contribute to the transition from traditional melon growing to biological.

Integrated time-series transcriptomic and metabolomic analyses reveal different inflammatory and adaptive immune responses contributing to host resistance to PRRSV.

Porcine reproductive and respiratory syndrome virus (PRRSV) is a highly contagious disease that affects the global pig industry. To understand mechanisms of susceptibility/resistance to PRRSV, this study profiled the time-serial white blood cells transcriptomic and serum metabolomic responses to PRRSV in piglets from a crossbred population of PRRSV-resistant Tongcheng pigs and PRRSV-susceptible Large White pigs. Gene set enrichment analysis (GSEA) illustrated that PRRSV infection up-regulated the expression levels of marker genes of dendritic cells, monocytes and neutrophils and inflammatory response, but down-regulated T cells, B cells and NK cells markers. CIBERSORT analysis confirmed the higher T cells proportion in resistant pigs during PRRSV infection. Resistant pigs showed a significantly higher level of T cell activation and lower expression levels of monocyte surface signatures post infection than susceptible pigs, corresponding to more severe suppression of T cell immunity and inflammatory response in susceptible pigs. Differentially expressed genes between resistant/susceptible pigs during the course of infection were significantly enriched in oxidative stress, innate immunity and humoral immunity, cell cycle, biotic stimulated cellular response, wounding response and behavior related pathways. Fourteen of these genes were distributed in 5 different QTL regions associated with PRRSV-related traits. Chemokine CXCL10 levels post PRRSV infection were differentially expressed between resistant pigs and susceptible pigs and can be a promising marker for susceptibility/resistance to PRRSV. Furthermore, the metabolomics dataset indicated differences in amino acid pathways and lipid metabolism between pre-infection/post-infection and resistant/susceptible pigs. The majority of metabolites levels were also down-regulated after PRRSV infection and were significantly positively correlated to the expression levels of marker genes in adaptive immune response. The integration of transcriptome and metabolome revealed concerted molecular events triggered by the infection, notably involving inflammatory response, adaptive immunity and G protein-coupled receptor downstream signaling. This study has increased our knowledge of the immune response differences induced by PRRSV infection and susceptibility differences at the transcriptomic and metabolomic levels, providing the basis for the PRRSV resistance mechanism and effective PRRS control.

The Impacts of Chlorine and Disinfection Byproducts on Antibiotic-Resistant Bacteria (ARB) and Their Conjugative Transfer

Antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) are emerging contaminants leading to severe worldwide health problems. Chlorination, a widely used procedure, was extensively explored as one of the main methods to remove ARB and ARGs in recent years. In this study, to enrich the analyses of chlorination, several comprehensive effects of the chlorine disinfection system on ARB and their conjugative transfer ability were explored. The results presented that the low dose of chlorine (<3-log inactivation rate) had little influence on the survival of bacteria in terms of their capacity for conjugative transfer and antibiotic resistance. The high dose of chlorine (>3-log inactivation rate) triggered cell membrane changes, with little influence on the bacteria in terms of their antibiotic resistance. However, their capacity for conjugative transfer sharply decreased. Minor consumption of chloramphenicol was achieved with the chlorine dose applied in the disinfection system. Monochloroacetonitrile (MCAN) had limited effects on the frequency of conjugative transfer, indicating that the existence of MCAN would not aggravate the dissemination of ARGs by conjugative transfer. The overall impacts of the chlorine disinfection system with different containments on antibiotic resistance need further investigation.

Giving children in rural India a voice during the Covid-19 pandemic: using drawing activities to understand children’s experiences and encourage global discussion

AimsRural communities and children are underrepresented in research. It is imperative that researchers engage with these populations, whether it be to disseminate findings or inform the research agenda. Post-pandemic, ensuring these communities are not forgotten is even more essential. Children may have more resistance to the virus but have had to endure the economic and social impact of the lockdowns. Art is used as a pressure-free way to engage children and elicit their experiences, feelings, and concerns.1 Therefore, we conducted a drawing activity with children living in rural areas to explore the pandemic from the child’s perspective. This examines how we worked with communities to use the arts to allow children who experience marginalisation to voice their feelings/opinions.MethodsPartnerships were built with local Indian community researchers and charities before commencing the work. The researchers understood the local context and spoke the primary local language. With the help of local teachers, the community researchers recruited 20 children from rural villages in Dungarpur to take part in the activity in a school hall. Trained community researchers explained the activity in the regional language, with translators who could speak the local dialect where necessary. Written consent was taken from both the parents and children, with agreement that the drawings could be freely used as part of exhibitions and presentations. Everyone wore masks and kept a minimum of 1 metre apart (the local guidance at the time) and hand sanitizer was readily available. Children were briefed on the project and, after some ice-breaker games, were asked to depict their experiences of the pandemic. The pictures were collected, scanned, and uploaded to our website as part of a global virtual exhibition on the arts and public health. The online event included talks from international scientists, artists, NGOs, and journalists.ResultsThe children’s drawings illustrated that they were worried about the global impact of the pandemic;felt isolated;feared for their parents’ job security;concerned about getting behind at school;and showed understanding of COVID safety precautions. Some depicted experiences of food insecurity. Others took a more positive approach, illustrating how happy they were about spending more time with their families. Presenting these images at the online exhibition raised awareness of the topic and stimulated discussion, potentially encouraging the multidisciplinary and influential attendees to pursue the research further or translate our approach to a different subject area. Additionally, it gave rural Indian communities a platform to voice their concernsConclusionThe engagement exercise allowed us to gauge the concerns of a marginalised community during the pandemic. Local partnerships and community collaborations were key to conducting this work. It allowed us to conduct an engagement activity that could be tailored to other contexts and which gave us a much-needed insight into the challenges that children in rural India have faced during the pandemic. This helped set research priorities and inform the discussion with policymakers, funders, and academics about key issues we need to address for children to rebuild their lives post pandemic.ReferenceChildren in Focus, J. Boyden.

Effect of HSP90AB1 on the replication of avian infectious bronchitis virus

The effects of heat shock protein HSPQOABl on the replication of avian infectious bronchitis virus(AIBV) were confirmed by using over expression and RNA interference methods. The results showed that over expression of HSPQOABI inhibited AIBV replication, whereas knockdown of HSPQOABl in- creased AIBV replication. These results indicated that HSPQOABI is a potential anti-viral host factor. These findings provide the basis for further study of the pathogenic mechanism of AIBV and anti-coronavirus infection.

Incidence and Severity of Aphid-transmitted Viruses and Horticultural Performance of Habanero Pepper (Capsicum chinense Jacq.) Breeding Lines in Benin

Habanero (Capsicum chinense Jacq.) is widely grown and consumed in West and Central African countries, and viral diseases represent an important production challenge. Diagnosis of the viral species affecting habanero productivity in Benin is limited, and understanding this will enable more efficient host resistance breeding. During 2019 and 2020, we characterized the incidence and severity of the viral diseases infecting nine promising habanero breeding lines and one commercial hybrid check under open field conditions in Benin. The horticultural performance, including yield and yield component traits of the entries, was determined during the 2 years of the experiment. A randomized complete block design was used with three replications, each with 24 plants. Data were recorded on days to 50% flowering and 50% fruit maturity, yield and on the yield components of fruit weight (g), fruit length (cm), and fruit width (mm), as well as disease incidence and severity. In total, 35 leaf samples were collected for viral diagnosis among habanero breeding lines. We found that Pepper veinal mottle virus (PVMV;Potyvirus) was the overwhelmingly predominant virus in our trials, with an 80% incidence;however, we found frequent coinfection of PVMV with Cucumber mosaic virus (CMV, Cucumovirus), Polerovirus, and, to a lesser extent, Chili veinal mottle virus (ChiVMV;Potyvirus). The mean disease incidence across all entries was 60%. AVPP1932 and PBC 2010 had the lowest disease incidence (35% and 43%, respectively), whereas AVPP1929 had the highest (86%) disease incidence. The F1 hybrid check Afadja had the overall highest yield, with 30 t·ha-1, followed by AVPP1932, with 19 t·ha-1, both in 2019. There was a negative correlation between disease incidence and total yield (r 5 20.44;P < 0.001), supporting previous studies indicating that viral diseases are major production constraints for habanero in West Africa. This study provides insight regarding the need to improve habanero for resistance to aphid-transmitted viruses and develop integrated pest management strategies to limit losses in Benin. [ FROM AUTHOR] Copyright of HortScience is the property of American Society for Horticultural Science and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full . (Copyright applies to all s.)

Evaluating the contribution of synthetic fungicides to cereal plant health and CO2 reduction targets against the backdrop of the increasingly complex regulatory environment in Europe. (Special Issue: On the topic of plant health in a one health context.)

The global COVID pandemic has impacted the world in ways and at a scale that few could have predicted, with many industries severely disrupted. Despite this, crops were sown and harvested, food was produced and agriculture continued to function, albeit it with many logistical challenges. Plant health lies at the heart of preventing crop losses through a combination of varietal resistance and agronomic practices. In the case of foliar plant diseases in wheat, varietal resistance plays a key role, but the use of synthetic fungicides is essential to minimize crop losses. European arable production faces a dilemma: how to contribute and maintain global food supplies but at the same time decrease emissions of greenhouse gases (GHGs), reduce inputs potentially harmful to biodiversity, society and the environment while ensuring no more land is brought into production. Throughout history, major disruptions in society have led to big steps in agricultural innovation. Presently, the major disruptive forces in Europe are not just a result of the COVID pandemic but the increasingly urgent need to address climate change. Within the European Green Deal, the Farm-to-Fork strategy is in place to help achieve climate neutrality by 2050, aiming for a reduction of GHG emissions of 55% by 2030. To achieve this, there will need to be a major adjustment to how food is produced, a realignment in plant health strategies and accelerated innovation across the agricultural sector. This paper aims to evaluate how synthetic fungicides presently contribute to plant health (mainly wheat) and food production as well as the management of GHG emissions. In addition, it explores the future challenges and prospects for their positive contribution in achieving global food security alongside emerging innovative technologies.

Genome Editing Strategies to Protect Livestock from Viral Infections.

The livestock industry is constantly threatened by viral disease outbreaks, including infections with zoonotic potential. While preventive vaccination is frequently applied, disease control and eradication also depend on strict biosecurity measures. Clustered regularly interspaced palindromic repeats (CRISPR) and associated proteins (Cas) have been repurposed as genome editors to induce targeted double-strand breaks at almost any location in the genome. Thus, CRISPR/Cas genome editors can also be utilized to generate disease-resistant or resilient livestock, develop vaccines, and further understand virus-host interactions. Genes of interest in animals and viruses can be targeted to understand their functions during infection. Furthermore, transgenic animals expressing CRISPR/Cas can be generated to target the viral genome upon infection. Genetically modified livestock can thereby reduce disease outbreaks and decrease zoonotic threats.